ClearPeak 2x SYBR Master Mix, No ROX# is a specialized premix for real-time fluorescence quantitative qPCR reactions using the dye method (SYBR Green I). The core component EcoTaq DNA Polymerase is an antibody based hot start DNA polymerase that can be restored by heating at 95℃. It has many advantages such as strong specificity and high detection sensitivity, and is paired with an optimal buffer optimized for qPCR. The unique qPCR buffer system of this product, combined with hot start enzymes, effectively inhibits the production of non-specific products and significantly improves the amplification efficiency of qPCR. It is very suitable for high specificity and sensitivity qPCR reactions. This product is also suitable for qPCR rapid reaction program. Good standard curves can be obtained within a wide quantitative range, with accurate, reproducible, and reliable quantification of target genes.
 
# Instruments do not require ROX correction: LightCycler® 480, LightCycler® 96, LightCycler® 2.0, Bio-Rad iCyler iQ, iQ5, CFX96, etc.