NutriCulture Colorimetric Caspase-8 Activity Assay Kit
Activation of Caspase-8 initiates apoptosis in mammalian cells. The NutriCulture Colorimetric Caspase-8 Activity Assay Kit provides a simple and convenient means for assaying the activity of Caspase-8 that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-8 activity.
Overview
- Catalog number
- C8A100
- Pack size
- 100 tests
- Shipping
- Ship with blue/dry ice.
- Storage
- Store at -20°C.
Activation of Caspase-8 initiates apoptosis in mammalian cells. The NutriCulture Colorimetric Caspase-8 Activity Assay Kit provides a simple and convenient means for assaying the activity of Caspase-8 that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-8 activity.
Documents
Find a Certificate of AnalysisProduct has been cited by 2 publications
- Arch Pharm (2026) DOI: 10.1002/ardp.70291 View publication
- SSRN (2026) DOI: 10.2139/ssrn.6170082 View publication
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