NutriCulture RPMI 1640, also known as RPMI Medium, was originally developed by Moore and his co-workers in 1966 Roswell Park Memorial Institute to culture human leukemia cells in suspension and monolayer. NutriCulture RPMI 1640 Medium was formulated for use in a 5% carbon dioxide atmosphere and has since been found suitable for culture of a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. NutriCulture RPMI 1640 has also traditionally been used for the serum-free expansion of human lymphoid cells for karyotype analysis.
Each lot of NutriCulture RPMI 1640 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.1 micron filter.
Each lot of NutriCulture RPMI 1640 is tested to confirm the absence of bacterial, fungal, and mycoplasmal contamination.
 
Product has been cited by 13 publications
 
Research Square (2025) DOI: 10.21203/rs.3.rs-5767315/v1
International Journal of Secondary Metabolite (2025) DOI: 10.21448/ijsm.1553575
Turkish Journal of Biochemistry (2024) DOI: 10.1515/tjb-2024-0122
Biological Trace Element Research (2024) DOI: 10.1007/s12011-024-04425-9
Head & Neck (2024) DOI: 10.1002/hed.27803
European Journal of Pharmacology (2024) DOI: 10.1016/j.ejphar.2024.176592 
Biological Trace Element Research (2024) DOI: 10.1007/s12011-024-04090-y
Journal of Biomolecular Structure and Dynamics (2024) DOI: 10.1080/07391102.2023.2253906
Cell biology international (2023) DOI: 10.1002/cbin.12085
Chemistry & Biodiversity (2023) DOI: 10.1002/cbdv.202200886
Turkish Journal of Biochemistry (2023) DOI: 10.1515/tjb-2023-0042
Journal of Heterocyclic Chemistry (2023) DOI: 10.1002/jhet.4643
Journal of the Iranian Chemical Society (2022) DOI: 10.1007/s13738-022-02656-y