EcoPURE Total RNA Micro Kit is designed as a simple and convenient purification of high quality total RNA including small RNAs (e.g. microRNAs) from cultured cells and frozen or fresh tissues. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, hazardous phenol-chloroform extractions, β-mercaptoethanol, or time-consuming alcohol precipitation. The standard protocol lasts less than 10 minutes at room temperature and purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection.