EcoClone Basic
EcoClone Basic is a high-copy cloning Vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0001
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoClone Basic is a high-copy cloning Vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pUC19
Vector Information
Vector size: 2686 bp Promoter: lac promoter/operator Bacterial selection: Ampicillin Mammalian selection: None Reporter / tag: lacZα screening cassette Origin of replication: pUC/ColE1-type high-copy bacterial origin
Applications
Routine DNA cloning; Subcloning; Blue/white screening; Sequencing template preparation.
Multiple Cloning Site (MCS)
EcoRI - SacI - Acc65I/KpnI - XmaI/SmaI - BamHI - XbaI - SalI - AccI/HincII - PstI - SphI - HindIII
Important Note
For Research Use Only. Not for use in diagnostic procedures. The MCS lies within the lacZα region, enabling insertional inactivation-based blue/white screening in compatible host strains.
