EcoExpress GST-A
EcoExpress GST-A is a gst fusion bacterial expression vector, ampr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0005
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoExpress GST-A is a gst fusion bacterial expression vector, ampr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pGEX-4T-1
Vector Information
Vector size: 4969 bp Promoter: tac promoter Bacterial selection: Ampicillin Mammalian selection: None Reporter / tag: GST fusion tag; thrombin cleavage site Origin of replication: bacterial origin of replication
Applications
GST fusion protein expression; Glutathione affinity purification; GST pull-down assays; Protein-protein interaction studies.
Multiple Cloning Site (MCS)
BamHI - EcoRI - XmaI/SmaI - SalI/AccI - XhoI - NotI
Important Note
For Research Use Only. Not for use in diagnostic procedures. The insert must be cloned in-frame with GST for fusion expression. pGEX 5' and 3' sequencing primer regions are present on the supplied map.
