EcoExpress CMV-Neo
EcoExpress CMV-Neo is a mammalian expression vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0002
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoExpress CMV-Neo is a mammalian expression vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pcDNA3.1(+)
Vector Information
Vector size: 5428 bp Promoter: CMV immediate-early promoter/enhancer Bacterial selection: Ampicillin Mammalian selection: Neomycin/G418 Reporter / tag: None Origin of replication: bacterial ori; SV40 ori
Applications
Mammalian gene overexpression; Transient transfection; Generation of stable G418-selected cell lines; Protein function and signaling studies.
Multiple Cloning Site (MCS)
NheI - AflII - HindIII - Acc65I/KpnI - BamHI - BstXI - EcoRI - EcoRV - BstXI - NotI - XhoI - XbaI - ApaI
Important Note
For Research Use Only. Not for use in diagnostic procedures. Insert orientation, reading frame and stop-codon design should be confirmed for the intended expression construct.
