EcoClone Phagemid
EcoClone Phagemid is a cloning & sequencing phagemid vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0007
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoClone Phagemid is a cloning & sequencing phagemid vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pBluescript SK(+)
Vector Information
Vector size: 2958 bp Promoter: lac promoter/operator; T7 and T3 promoters flank the cloning region Bacterial selection: Ampicillin Mammalian selection: None Reporter / tag: lacZα screening cassette Origin of replication: bacterial ori; f1 ori
Applications
Routine cloning and subcloning; Blue/white screening; Sanger sequencing template preparation; In vitro transcription from T7/T3 promoters.
Multiple Cloning Site (MCS)
Acc65I/KpnI - ApaI - XhoI - SalI/AccI/HincII - ClaI - HindIII - EcoRV - EcoRI - PstI - XmaI/SmaI - BamHI - SpeI - XbaI - NotI - SacII - BstXI - SacI
Important Note
For Research Use Only. Not for use in diagnostic procedures. The supplied map shows T7 and T3 promoters, M13 forward/reverse primer regions, lacZα, f1 ori and AmpR.
