EcoExpress T7-HisA
EcoExpress T7-HisA is a n-terminal his-tag bacterial expression vector, ampr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0006
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoExpress T7-HisA is a n-terminal his-tag bacterial expression vector, ampr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pET-15b
Vector Information
Vector size: 5708 bp Promoter: T7 promoter / lac operator Bacterial selection: Ampicillin Mammalian selection: None Reporter / tag: N-terminal 6xHis; thrombin cleavage site Origin of replication: bacterial origin of replication
Applications
Recombinant protein expression; His-tag affinity purification; Enzyme production; Protein characterization.
Multiple Cloning Site (MCS)
NcoI - NdeI - XhoI - BamHI - HindIII
Important Note
For Research Use Only. Not for use in diagnostic procedures. The supplied map shows the 6xHis region, thrombin site, T7 promoter/lac operator and T7 terminator. Insert reading frame and cleavage strategy should be confirmed before cloning.
