EcoPlant GUS
EcoPlant GUS is a gus plant binary expression vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0008
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoPlant GUS is a gus plant binary expression vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pBI121
Vector Information
Vector size: 14758 bp Promoter: CaMV 35S promoter; NOS promoter Bacterial selection: Kanamycin resistance; tetracycline resistance shown on map Mammalian selection: Not applicable Reporter / tag: GUS (β-glucuronidase) reporter; NeoR/KanR plant selection cassette Origin of replication: oriV; bacterial replication/transfer elements
Applications
Agrobacterium-mediated plant transformation; Plant gene expression studies; GUS reporter assays; Plant promoter / transgene studies.
Multiple Cloning Site (MCS)
HindIII - SbfI - XbaI - BamHI - XmaI/SmaI (cloning-region sites shown near the CaMV 35S/GUS cassette)
Important Note
For Research Use Only. Not for use in diagnostic procedures. The supplied map shows RB/LB T-DNA repeats, CaMV 35S-driven GUS, NOS regulatory elements and a NeoR/KanR selection cassette within the T-DNA region. Vector behavior and selection conditions should be validated for the intended plant/Agrobacterium system.
